Review



isotonic mitochondrial isolation buffer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher isotonic mitochondrial isolation buffer
    Isotonic Mitochondrial Isolation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolation+buffer/Sucrose/pm41957015-338-41-66
    Average 99 stars, based on 1 article reviews
    isotonic mitochondrial isolation buffer - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Impact of APOE on cerebrovascular lipid profile in Alzheimer's disease.
    Article Snippet: .. After the removal of meninges with tweezers, we thawed each temporal cortex sample (400 mg) on ice in vascular isolation buffer (10 μM HEPES (Thermo Fisher Scientific, Cat No. 15630080, Waltham, MA, USA) in HBSS (Thermo Fisher Scientific, Cat No. 14025092)). ..

    Article Title: RNA-mediated condensation of TFE3 oncofusions facilitates transcriptional hub formation to promote translocation renal cell carcinoma.
    Article Snippet: UOK109 cells were transfected with 500 ng of enCas12a (Addgene # 89351) along with 500 ng of purified PCR cassettes. .. After fixation, the cell pellets were washed three times with ice-cold PBS and resuspended in 1× isolation buffer (3.2M Sucrose, 10 Tris-HCl pH 7.5, 5mMMgCl2, 1% Triton X-100) supplemented with 100U/mL RNAseOUT (Invitrogen, 10777019), followed by incubation on ice for 20min. ..

    Article Title: Novel Binding Partners of the Vacuolar Transporter Chaperone (VTC) complex in Acidocalcisomes of Leishmania tarentolae
    Article Snippet: .. Cells were collected by centrifugation at 2,000 × g for 3 min, and the pellet (∼2 g wet weight) was washed with cold isolation buffer (125 mM sucrose, 50 mM KCl, 4 mM MgCl2, 0.5 mM EDTA, 5 mM DTT, 20 mM Hepes-KOH, pH 7.2) supplemented with PierceTM Protease Inhibitor Mini Tablets, EDTA-free (Thermo Scientific, A32955). ..

    Article Title: RNA-mediated condensation of TFE3 oncofusions facilitates transcriptional hub formation to promote translocation renal cell carcinoma
    Article Snippet: .. After fixation, the cell pellets were washed three times with ice-cold PBS and resuspended in 1× isolation buffer (3.2 M Sucrose, 10 Tris-HCl pH 7.5, 5 mM MgCl2, 1% Triton X-100) supplemented with 100 U/mL RNAseOUT (Invitrogen, 10777019), followed by incubation on ice for 20 min. ..

    Article Title: Pre-clinical therapeutics for osteopetrosis caused by Plekhm1 deficiency using ex vivo and in vivo gene therapies
    Article Snippet: .. Harvest and purification of lineage—negative (Lin - ) hematopoietic stem cells (HSCs) from bone marrow Whole bone marrow cells (BMCs) were flushed from lower limbs of wild-type mice carrying green fluorescent protein (GFP) (8 weeks, 20 ± 2 g) with a syringe filled with isolation buffer (Phosphate Buffer Saline [PBS] supplemented with 2% fetal bovine serum [FBS] [Gibco BRL, Gaithersburg, MD, USA]). ..

    Article Title: Immunomediator expression in human periodontal ligament MSCs varies depending on surface CD146 expression.
    Article Snippet: Bovine serum albumin (BSA; Capricorn Scientific GmbH, Ebsdorfergrund, Germany) was added to reach a final BSA concentration of 1 mg/ml for storage at 4° Celsius. .. The antibody concentration was estimated to be 0.42 mg/ml using the following equation: inital mg of antibody * 0.85 ml in collection tube = mg mlDSBXTM biotin labeled antibody For the depletion and enrichment of CD146+ hPDL-MSCs (Fig. 1b), 7.9x107 FlowCompTM Dynabeads® with an initial concentration of ~ 1.5x109 beads/ml were washed with isolation buffer (Ca2+ and Mg2+-free phosphate buffer saline buffer (Gibco, Carlsbad, CA, USA) complemented with 2mM ethylendiamintetraacetat (Merck Millipore, Burlington, VT, USA) and 2% FBS). ..

    Article Title: Immunomediator expression in human periodontal ligament MSCs varies depending on surface CD146 expression
    Article Snippet: Bovine serum albumin (BSA; Capricorn Scientific GmbH, Ebsdorfergrund, Germany) was added to reach a final BSA concentration of 1 mg/ml for storage at 4 °C. .. The antibody concentration was estimated to be 0.42 mg/ml using the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\:\frac{inital\:mg\:of\:antibody\:*\:0.85}{ml\:in\:collection\:tube}=mg/mlDSBX^{\mathrm{TM}}\:biotin\:labeled\:antibody$$\end{document} For the depletion and enrichment of CD146 + hPDL-MSCs (see Supplementary Fig. b), 7.9 × 10 7 FlowCompTM Dynabeads ® with an initial concentration of ~ 1.5 × 10 9 beads/ml were washed with isolation buffer (Ca 2+ and Mg 2+ -free phosphate buffer saline buffer (Gibco, Carlsbad, CA, USA) complemented with 2mM ethylendiamintetraacetat (Merck Millipore, Burlington, VT, USA) and 2% FBS). ..

    Article Title: Impact of APOE on cerebrovascular lipid profile in Alzheimer’s disease
    Article Snippet: .. After the removal of meninges with tweezers, we thawed each temporal cortex sample (400 mg) on ice in vascular isolation buffer (10 μM HEPES (Thermo Fisher Scientific, Cat No. 15630080, Waltham, MA, USA) in HBSS (Thermo Fisher Scientific, Cat No. 14025092)). ..

    Incubation:

    Article Title: RNA-mediated condensation of TFE3 oncofusions facilitates transcriptional hub formation to promote translocation renal cell carcinoma.
    Article Snippet: UOK109 cells were transfected with 500 ng of enCas12a (Addgene # 89351) along with 500 ng of purified PCR cassettes. .. After fixation, the cell pellets were washed three times with ice-cold PBS and resuspended in 1× isolation buffer (3.2M Sucrose, 10 Tris-HCl pH 7.5, 5mMMgCl2, 1% Triton X-100) supplemented with 100U/mL RNAseOUT (Invitrogen, 10777019), followed by incubation on ice for 20min. ..

    Article Title: RNA-mediated condensation of TFE3 oncofusions facilitates transcriptional hub formation to promote translocation renal cell carcinoma
    Article Snippet: .. After fixation, the cell pellets were washed three times with ice-cold PBS and resuspended in 1× isolation buffer (3.2 M Sucrose, 10 Tris-HCl pH 7.5, 5 mM MgCl2, 1% Triton X-100) supplemented with 100 U/mL RNAseOUT (Invitrogen, 10777019), followed by incubation on ice for 20 min. ..

    Centrifugation:

    Article Title: Novel Binding Partners of the Vacuolar Transporter Chaperone (VTC) complex in Acidocalcisomes of Leishmania tarentolae
    Article Snippet: .. Cells were collected by centrifugation at 2,000 × g for 3 min, and the pellet (∼2 g wet weight) was washed with cold isolation buffer (125 mM sucrose, 50 mM KCl, 4 mM MgCl2, 0.5 mM EDTA, 5 mM DTT, 20 mM Hepes-KOH, pH 7.2) supplemented with PierceTM Protease Inhibitor Mini Tablets, EDTA-free (Thermo Scientific, A32955). ..

    Protease Inhibitor:

    Article Title: Novel Binding Partners of the Vacuolar Transporter Chaperone (VTC) complex in Acidocalcisomes of Leishmania tarentolae
    Article Snippet: .. Cells were collected by centrifugation at 2,000 × g for 3 min, and the pellet (∼2 g wet weight) was washed with cold isolation buffer (125 mM sucrose, 50 mM KCl, 4 mM MgCl2, 0.5 mM EDTA, 5 mM DTT, 20 mM Hepes-KOH, pH 7.2) supplemented with PierceTM Protease Inhibitor Mini Tablets, EDTA-free (Thermo Scientific, A32955). ..

    Purification:

    Article Title: Pre-clinical therapeutics for osteopetrosis caused by Plekhm1 deficiency using ex vivo and in vivo gene therapies
    Article Snippet: .. Harvest and purification of lineage—negative (Lin - ) hematopoietic stem cells (HSCs) from bone marrow Whole bone marrow cells (BMCs) were flushed from lower limbs of wild-type mice carrying green fluorescent protein (GFP) (8 weeks, 20 ± 2 g) with a syringe filled with isolation buffer (Phosphate Buffer Saline [PBS] supplemented with 2% fetal bovine serum [FBS] [Gibco BRL, Gaithersburg, MD, USA]). ..

    Saline:

    Article Title: Pre-clinical therapeutics for osteopetrosis caused by Plekhm1 deficiency using ex vivo and in vivo gene therapies
    Article Snippet: .. Harvest and purification of lineage—negative (Lin - ) hematopoietic stem cells (HSCs) from bone marrow Whole bone marrow cells (BMCs) were flushed from lower limbs of wild-type mice carrying green fluorescent protein (GFP) (8 weeks, 20 ± 2 g) with a syringe filled with isolation buffer (Phosphate Buffer Saline [PBS] supplemented with 2% fetal bovine serum [FBS] [Gibco BRL, Gaithersburg, MD, USA]). ..

    Article Title: Immunomediator expression in human periodontal ligament MSCs varies depending on surface CD146 expression.
    Article Snippet: Bovine serum albumin (BSA; Capricorn Scientific GmbH, Ebsdorfergrund, Germany) was added to reach a final BSA concentration of 1 mg/ml for storage at 4° Celsius. .. The antibody concentration was estimated to be 0.42 mg/ml using the following equation: inital mg of antibody * 0.85 ml in collection tube = mg mlDSBXTM biotin labeled antibody For the depletion and enrichment of CD146+ hPDL-MSCs (Fig. 1b), 7.9x107 FlowCompTM Dynabeads® with an initial concentration of ~ 1.5x109 beads/ml were washed with isolation buffer (Ca2+ and Mg2+-free phosphate buffer saline buffer (Gibco, Carlsbad, CA, USA) complemented with 2mM ethylendiamintetraacetat (Merck Millipore, Burlington, VT, USA) and 2% FBS). ..

    Article Title: Immunomediator expression in human periodontal ligament MSCs varies depending on surface CD146 expression
    Article Snippet: Bovine serum albumin (BSA; Capricorn Scientific GmbH, Ebsdorfergrund, Germany) was added to reach a final BSA concentration of 1 mg/ml for storage at 4 °C. .. The antibody concentration was estimated to be 0.42 mg/ml using the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\:\frac{inital\:mg\:of\:antibody\:*\:0.85}{ml\:in\:collection\:tube}=mg/mlDSBX^{\mathrm{TM}}\:biotin\:labeled\:antibody$$\end{document} For the depletion and enrichment of CD146 + hPDL-MSCs (see Supplementary Fig. b), 7.9 × 10 7 FlowCompTM Dynabeads ® with an initial concentration of ~ 1.5 × 10 9 beads/ml were washed with isolation buffer (Ca 2+ and Mg 2+ -free phosphate buffer saline buffer (Gibco, Carlsbad, CA, USA) complemented with 2mM ethylendiamintetraacetat (Merck Millipore, Burlington, VT, USA) and 2% FBS). ..

    Concentration Assay:

    Article Title: Immunomediator expression in human periodontal ligament MSCs varies depending on surface CD146 expression.
    Article Snippet: Bovine serum albumin (BSA; Capricorn Scientific GmbH, Ebsdorfergrund, Germany) was added to reach a final BSA concentration of 1 mg/ml for storage at 4° Celsius. .. The antibody concentration was estimated to be 0.42 mg/ml using the following equation: inital mg of antibody * 0.85 ml in collection tube = mg mlDSBXTM biotin labeled antibody For the depletion and enrichment of CD146+ hPDL-MSCs (Fig. 1b), 7.9x107 FlowCompTM Dynabeads® with an initial concentration of ~ 1.5x109 beads/ml were washed with isolation buffer (Ca2+ and Mg2+-free phosphate buffer saline buffer (Gibco, Carlsbad, CA, USA) complemented with 2mM ethylendiamintetraacetat (Merck Millipore, Burlington, VT, USA) and 2% FBS). ..

    Article Title: Immunomediator expression in human periodontal ligament MSCs varies depending on surface CD146 expression
    Article Snippet: Bovine serum albumin (BSA; Capricorn Scientific GmbH, Ebsdorfergrund, Germany) was added to reach a final BSA concentration of 1 mg/ml for storage at 4 °C. .. The antibody concentration was estimated to be 0.42 mg/ml using the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\:\frac{inital\:mg\:of\:antibody\:*\:0.85}{ml\:in\:collection\:tube}=mg/mlDSBX^{\mathrm{TM}}\:biotin\:labeled\:antibody$$\end{document} For the depletion and enrichment of CD146 + hPDL-MSCs (see Supplementary Fig. b), 7.9 × 10 7 FlowCompTM Dynabeads ® with an initial concentration of ~ 1.5 × 10 9 beads/ml were washed with isolation buffer (Ca 2+ and Mg 2+ -free phosphate buffer saline buffer (Gibco, Carlsbad, CA, USA) complemented with 2mM ethylendiamintetraacetat (Merck Millipore, Burlington, VT, USA) and 2% FBS). ..

    Labeling:

    Article Title: Immunomediator expression in human periodontal ligament MSCs varies depending on surface CD146 expression.
    Article Snippet: Bovine serum albumin (BSA; Capricorn Scientific GmbH, Ebsdorfergrund, Germany) was added to reach a final BSA concentration of 1 mg/ml for storage at 4° Celsius. .. The antibody concentration was estimated to be 0.42 mg/ml using the following equation: inital mg of antibody * 0.85 ml in collection tube = mg mlDSBXTM biotin labeled antibody For the depletion and enrichment of CD146+ hPDL-MSCs (Fig. 1b), 7.9x107 FlowCompTM Dynabeads® with an initial concentration of ~ 1.5x109 beads/ml were washed with isolation buffer (Ca2+ and Mg2+-free phosphate buffer saline buffer (Gibco, Carlsbad, CA, USA) complemented with 2mM ethylendiamintetraacetat (Merck Millipore, Burlington, VT, USA) and 2% FBS). ..



    Similar Products

    99
    Thermo Fisher isotonic mitochondrial isolation buffer
    Isotonic Mitochondrial Isolation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolation+buffer/Sucrose/pm41957015-338-41-66
    Average 99 stars, based on 1 article reviews
    isotonic mitochondrial isolation buffer - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    10X Genomics chromium nuclei isolation kit lysis buffer
    Chromium Nuclei Isolation Kit Lysis Buffer, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolation+buffer/pm42265312-272-6-4
    Average 86 stars, based on 1 article reviews
    chromium nuclei isolation kit lysis buffer - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    MACHEREY NAGEL nucleospin viral rna isolation kit
    Nucleospin Viral Rna Isolation Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolation+buffer/NucleoSpin+RNA+%2F+DNA+Buffer+Set+for+parallel+RNA+and+DNA+purification/pmc13157107-84-9-14
    Average 96 stars, based on 1 article reviews
    nucleospin viral rna isolation kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    95
    Miltenyi Biotec ice cold storage buffer
    Ice Cold Storage Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolation+buffer/Mitochondria+Isolation+Kit%2C+human/10__1096_slash_fj__202500435r-73-14-20
    Average 95 stars, based on 1 article reviews
    ice cold storage buffer - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Miltenyi Biotec storage buffer
    Storage Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolation+buffer/Mitochondria+Isolation+Kit%2C+human/10__1096_slash_fj__202500435r-74-8-12
    Average 95 stars, based on 1 article reviews
    storage buffer - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results